Lactose at the extremely high concentration of 200 g l −l did not induce the drastic morphological changes observed in the case of 200 g l -1 of yeast extract. At 200 g l −l of lactose the production of lovastatin, (+)-geodin and asterric acid was visibly stimulated compared to the media containing 0.2, 2 and 20 g l −l of the sugar substrate, but at the same time no traces of butyrolactone I could be detected in the broth. Under these conditions the biosynthesis of (+)-geodin and asterric acid was totally blocked, lovastatin formation was found to be at a relatively low level and biomass production turned out to be greater than in the remaining variants, where the pelleted growth was observed. At 200 g l −l of yeast extract clumps and loose hyphae were recorded instead of pellets commonly observed in submerged cultures of A. ![]() The morphological differences of mycelial objects were quantified with the use of morphological parameters calculated by applying the tools of digital image analysis. Evaluation of morphology and secondary metabolites production in Aspergillus terreus ATCC 20542 cultures over a wide range of lactose and yeast extract concentrations from 0.2 up to an extremely high level of 200 g l −l.
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